Fig. 1

Evaluation of human, small intestinal monolayer systems. a Schematic and fluorescence side images of the gradient cross-linked scaffold (top) and conventional scaffold overlaying a porous membrane (bottom). Scale bar = 300 μm. b Fluorescence images of the monolayers at day 10 stained for EdU (green), ALP (red), Muc2 (yellow), and nuclei (blue). Scale bar = 100 μm. c Quantification of Edu, ALP, and Muc2 on day 5 and day 10 of culture as a percentage of the Hoechst 33342 (nuclei stain) positive area (n = 3). Edu, ALP, and Muc2 expression was not statistically different between the two monolayer systems in EM or DM