Fig. 3

Evaluation of barrier function and monolayer integrity. a TEER measurements over 18 days in both monolayer culture systems (n = 3). b LY permeability values (n = 3) and c) fluorescein-dextran permeability values (n = 3) through the monolayers on the gradient cross-linked scaffold and conventional scaffold compared to the permeability of LY and fluorescein-dextran of the gradient cross-linked scaffold in the absence of cells (n = 3) and conventional scaffold in the absence of cells (n = 3) on day 10 of culture. The LY and fluorescein-dextran permeability values through the gradient cross-linked scaffold and conventional scaffold in the absence of cells were not statistically different