Fig. 2
From: Non-canonical amino acid labeling in proteomics and biotechnology
Incorporation of ncAAs by native cellular machinery. Non-canonical amino acids (ncAAs) are incorporated into the growing polypeptide chain as the protein is synthesized at the ribosome. a ncAA is covalently attached to a tRNA by aminoacyl tRNA synthetase (aaRS). b The tRNA, charged with the ncAA (ncAA-tRNA, ncAA in blue), recognizes mRNA codons in the ribosome and the ncAA is added to the growing polypeptide chain