Fig. 4
From: Non-canonical amino acid labeling in proteomics and biotechnology
Overview of residue-specific protein labeling. a A ncAA (red sphere) is added to the system (cell culture or animal model). Native translational machinery incorporates the ncAA into the newly synthesized proteins. b An example of the codon sequence and corresponding peptides that result from either natural synthesis or synthesis in the presence of the ncAA. c A peptide labeled at two residue-specific sites with a ncAA carrying an alkyne functional group is conjugated to a azide-containing fluorophore via CuAAC