From: Non-canonical amino acid labeling in proteomics and biotechnology
ncAA | Codon replacement | Natural amino acid analog | Genetic modification | Labeling method |
---|---|---|---|---|
Hag | AUG | Met | Not neededa | |
Hpg | AUG | Met | Not neededa | |
Aha | AUG | Met | Not neededa | |
Anl | AUG | Met | Transgenic lines that express a MetRS mutant capable of charging Anlb | |
Azf | UUC, UUU UAG | Phe | Transgenic lines that express a PheRS mutant capable of charging Azfb Transgenic lines that express an orthogonal aaRS/amber suppressor tRNA pair evolved for Azf specificity | Residue-specific [55] Site-specific [118] |
Acf | UUC, UUU UAG | Phe | Transgenic lines that express a PheRS mutant capable of charging Acfb Transgenic lines that express an orthogonal aaRS/amber suppressor tRNA pair evolved for Acf specificity | Residue-specific [136] Site-specific [111] |
Pxf | UAG | Tyr | Transgenic lines that express an orthogonal aaRS/amber suppressor tRNA pair evolved for Pxf specificity | Site-specific [124] |