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Table 2 Online quality assessment of human CD4+ and CD8+ T cells

From: Novel biomanufacturing platform for large-scale and high-quality human T cells production

Functiona,b,c

Markers

Day − 4d

Day 0

Day 2

Day 4

Day 8 (re-stimulated)

CD4+

CD8+

CD4+

CD8+

CD4+

CD8+

CD4+

CD8+

CD4+

CD8+

Cell type

CD4+

99.1 ± 0.5

0.7 ± 0.0

99.0 ± 1.1

0.8 ± 0.0

99.6 ± 0.2

0.6 ± 0.3

99.6 ± 0.4

0.4 ± 0.2

98.6 ± 1.1

0.3 ± 0.0

CD8+

0.8 ± 0.1

99.5 ± 0.0

0.4 ± 0.3

99.8 ± 0.0

0.3 ± 0.1

99.3 ± 0.0

0.4 ± 0.4

93.8 ± 0.0

0.4 ± 0.1

99.0 ± 0.1

Activation signal

CD3+ (stimulation)

97.8 ± 2.6

98.2 ± 2.2

97.5 ± 2.8

92.6 ± 8.4

99.4 ± 0.7

96.3 ± 4.1

98.3 ± 2.3

91.7 ± 7.7

95.1 ± 3.7

96.8 ± 0.5

ICOS+/CD278+ (co-stimulation)

30.3 ± 19.9

13.3 ± 10.8

99.6 ± 0.1

99.5 ± 0.6

99.7 ± 0.2

99.2 ± 1.0

99.5 ± 0.6

91.2 ± 12.7

99.8 ± 0.2

99.8 ± 0.2

OX40+/CD134+ (co-stimulatione)

1.8 ± 1.3

0.1 ± 0.1

2.7 ± 1.6

1.7 ± 1.3

0.2 ± 0.2

0.1 ± 0.1

0.3 ± 0.5

0.1 ± 0.1

0.4 ± 0.3

0.0 ± 0.0

CD27+ (co-stimulation)

83.3 ± 2.7

64.1 ± 5.1

83.1 ± 9.9

95.4 ± 1.1

87.9 ± 4.7

86.6 ± 4.0

92.3 ± 3.4

89.7 ± 1.3

67.2 ± 2.4

88.5 ± 1.7

Inhibitory signal

PD-1+/CD279+ (TCR signaling)

5.7 ± 4.3

6.5 ± 5.6

84.5 ± 4.3

84.7 ± 4.3

30.3 ± 24.6

7.0 ± 3.8

8.1 ± 3.9

3.3 ± 2.5

63.1 ± 6.0

67.9 ± 15.6

LAG-3+/CD223+

95.0 ± 6.2

97.6 ± 2.8

23.3 ± 11.9

61.6 ± 15.8

11.0 ± 5.8

24.0 ± 12.7

4.4 ± 1.2

4.9 ± 4.0

16.4 ± 8.6

47.4 ± 8.6

KLRG1+

0.9 ± 0.4

1.4 ± 0.7

5.5 ± 2.5

7.9 ± 2.3

7.5 ± 0.7

6.6 ± 1.0

6.7 ± 2.3

6.4 ± 3.4

6.4 ± 1.5

4.7 ± 0.4

Memory typef

CCR7+ (central memory T cell)

19.4 ± 7.6

6.0 ± 1.0

90.7 ± 6.6

86.5 ± 8.0

78.3 ± 5.8

70.1 ± 5.2

81.7 ± 5.5

68.5 ± 6.4

39.1 ± 9.9

29.2 ± 7.2

CD45RO+ (memory T cell)

86.7 ± 4.0

57.4 ± 13.6

97.0 ± 4.4

99.7 ± 0.4

98.4 ± 1.9

93.5 ± 7.8

98.6 ± 0.9

94.1 ± 2.6

99.6 ± 0.3

99.0 ± 0.1

CD45RA+ (naïve T cell)

13.9 ± 3.0

55.3 ± 6.6

2.4 ± 1.1

3.5 ± 2.6

3.7 ± 2.3

3.6 ± 2.4

0.7 ± 0.3

1.1 ± 0.7

1.9 ± 1.5

3.4 ± 0.9

  1. Notes
  2. aThe CD4+ T cells and CD8+ T cells were cultivated in single culture. The T cells used for data collection came from 3 donors.
  3. bFlow cytometry was used to access T cell quality and the expression of these markers was reported as the binding intensity of antibodies.
  4. cAll data are presented as mean ± standard error of the mean (SEM).
  5. dDay-4 data was collected from isolated cells without stimulation. Day 8 data was collected after 2nd stimulation.
  6. eCo-stimulation could happen with various mechanisms25. The low values of OX40/CD134 indicated the low possibility of this receptor correlated co-stimulation mechanism.
  7. fAll cells obtained a memory phenotype after stimulation.
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Journal of Biological Engineering

ISSN: 1754-1611