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Table 2 Online quality assessment of human CD4+ and CD8+ T cells

From: Novel biomanufacturing platform for large-scale and high-quality human T cells production

Functiona,b,c Markers Day − 4d Day 0 Day 2 Day 4 Day 8 (re-stimulated)
CD4+ CD8+ CD4+ CD8+ CD4+ CD8+ CD4+ CD8+ CD4+ CD8+
Cell type CD4+ 99.1 ± 0.5 0.7 ± 0.0 99.0 ± 1.1 0.8 ± 0.0 99.6 ± 0.2 0.6 ± 0.3 99.6 ± 0.4 0.4 ± 0.2 98.6 ± 1.1 0.3 ± 0.0
CD8+ 0.8 ± 0.1 99.5 ± 0.0 0.4 ± 0.3 99.8 ± 0.0 0.3 ± 0.1 99.3 ± 0.0 0.4 ± 0.4 93.8 ± 0.0 0.4 ± 0.1 99.0 ± 0.1
Activation signal CD3+ (stimulation) 97.8 ± 2.6 98.2 ± 2.2 97.5 ± 2.8 92.6 ± 8.4 99.4 ± 0.7 96.3 ± 4.1 98.3 ± 2.3 91.7 ± 7.7 95.1 ± 3.7 96.8 ± 0.5
ICOS+/CD278+ (co-stimulation) 30.3 ± 19.9 13.3 ± 10.8 99.6 ± 0.1 99.5 ± 0.6 99.7 ± 0.2 99.2 ± 1.0 99.5 ± 0.6 91.2 ± 12.7 99.8 ± 0.2 99.8 ± 0.2
OX40+/CD134+ (co-stimulatione) 1.8 ± 1.3 0.1 ± 0.1 2.7 ± 1.6 1.7 ± 1.3 0.2 ± 0.2 0.1 ± 0.1 0.3 ± 0.5 0.1 ± 0.1 0.4 ± 0.3 0.0 ± 0.0
CD27+ (co-stimulation) 83.3 ± 2.7 64.1 ± 5.1 83.1 ± 9.9 95.4 ± 1.1 87.9 ± 4.7 86.6 ± 4.0 92.3 ± 3.4 89.7 ± 1.3 67.2 ± 2.4 88.5 ± 1.7
Inhibitory signal PD-1+/CD279+ (TCR signaling) 5.7 ± 4.3 6.5 ± 5.6 84.5 ± 4.3 84.7 ± 4.3 30.3 ± 24.6 7.0 ± 3.8 8.1 ± 3.9 3.3 ± 2.5 63.1 ± 6.0 67.9 ± 15.6
LAG-3+/CD223+ 95.0 ± 6.2 97.6 ± 2.8 23.3 ± 11.9 61.6 ± 15.8 11.0 ± 5.8 24.0 ± 12.7 4.4 ± 1.2 4.9 ± 4.0 16.4 ± 8.6 47.4 ± 8.6
KLRG1+ 0.9 ± 0.4 1.4 ± 0.7 5.5 ± 2.5 7.9 ± 2.3 7.5 ± 0.7 6.6 ± 1.0 6.7 ± 2.3 6.4 ± 3.4 6.4 ± 1.5 4.7 ± 0.4
Memory typef CCR7+ (central memory T cell) 19.4 ± 7.6 6.0 ± 1.0 90.7 ± 6.6 86.5 ± 8.0 78.3 ± 5.8 70.1 ± 5.2 81.7 ± 5.5 68.5 ± 6.4 39.1 ± 9.9 29.2 ± 7.2
CD45RO+ (memory T cell) 86.7 ± 4.0 57.4 ± 13.6 97.0 ± 4.4 99.7 ± 0.4 98.4 ± 1.9 93.5 ± 7.8 98.6 ± 0.9 94.1 ± 2.6 99.6 ± 0.3 99.0 ± 0.1
CD45RA+ (naïve T cell) 13.9 ± 3.0 55.3 ± 6.6 2.4 ± 1.1 3.5 ± 2.6 3.7 ± 2.3 3.6 ± 2.4 0.7 ± 0.3 1.1 ± 0.7 1.9 ± 1.5 3.4 ± 0.9
  1. Notes
  2. aThe CD4+ T cells and CD8+ T cells were cultivated in single culture. The T cells used for data collection came from 3 donors.
  3. bFlow cytometry was used to access T cell quality and the expression of these markers was reported as the binding intensity of antibodies.
  4. cAll data are presented as mean ± standard error of the mean (SEM).
  5. dDay-4 data was collected from isolated cells without stimulation. Day 8 data was collected after 2nd stimulation.
  6. eCo-stimulation could happen with various mechanisms25. The low values of OX40/CD134 indicated the low possibility of this receptor correlated co-stimulation mechanism.
  7. fAll cells obtained a memory phenotype after stimulation.