Fig. 2

Schematic representation of the modified B18R mRNA synthesis. The coding sequence (CDS) of B18R was amplified from the pcDNA 3.3 vector using PCR. The amplified DNA was purified and the quality was examined by the detection of a single DNA band of approximately 1400 bases. Afterwards, the amplified DNA was used as template for the in vitro transcription (IVT) to generate the B18R mRNA. After the IVT, the transcribed mRNA was dephosphorylated, purified, and the quality of mRNA was proved using 1% agarose gel electrophoresis and the detection of one clear band with about 1400 bases