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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Efficient reduction of synthetic mRNA induced immune activation by simultaneous delivery of B18R encoding mRNA

Fig. 4

Investigation of eGFP expression after the co-transfection of fibroblasts with eGFP mRNA and different amounts of B18R mRNA using flow cytometry. 1 × 105 fibroblasts were transfected for two following days with 1.5 μg eGFP alone or with 0.2, 0.5, 1, or 1.5 μg B18R mRNA. Cells treated with only Opti-MEM or Opti-MEM and the transfection reagent Lipofectamine® 2000 served as negative controls. The eGFP expression was analyzed 24 h after (a) the first transfection and (b) the second transfection by flow cytometry. Results are presented as means ± SD (n = 3). Differences were analyzed using one-way ANOVA following Bonferroni’s multiple comparison test. (***p < 0.001, ****p < 0.0001). ns: not significant

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