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Table 3 Strains and plasmids used and constructed in this study

From: Elucidation of the biosynthesis pathway and heterologous construction of a sustainable route for producing umbelliferone

  Description Source
Strains
E.coli DH5a General cloning host Invitrogen
E. coli K12 (MG1655) F-, λ-, ilvG-, rfb-50, rph-1 Novagen
E.coli BL21(DE3) F-, ompT, hsdS (rB mB), gal, dcm (DE3) Novagen
 K12 E. coli K12 carrying the gene for T7 RNA polymerase in pGEX6P-1 This study
 P K12ΔpheA This study
 E K12ΔtrpE This study
 PE K12ΔpheAΔtrpE This study
 PET K12ΔpheAΔtrpEΔtyrR This study
 PET-pRSF PET carrying pRSF This study
 K12-pCDF K12 carrying pCDF This study
 P-pCDF P carrying pCDF This study
 E-pCDF E carrying pCDF This study
 PE-pCDF PE carrying pCDF This study
 PET-pCDF PET carrying pCDF This study
 PET-pRSF-pCDF PET-pRSF carrying pCDF This study
 PET-pRSF-Pc4CL1 PET-pRSF carrying pCDF-Pc4CL1 This study
 PET-pRSF-Pt4CL PET-pRSF carrying pCDF-Pt4CL This study
 PET-pRSF-At4CL1 PET-pRSF carrying pCDF-At4CL1 This study
 PET-pRSF-At4CL2 PET-pRSF carrying pCDF-At4CL2 This study
 PET-pRSF-Pc4CL1-M PET-pRSF carrying pCDF-Pc4CL1 (Q272H/F267 L) This study
Plasmids
 pET28a T7 promoters, pBR322 ori, KnR Novagen
 pRSFDuet-1 Double T7 promoters, RSF ori, KnR Novagen
 pCDFDuet-1 Double T7 promoters, CloDF13 ori, SmR Novagen
 pRSF-1 pRSFDuet-1 carrying tktA This study
 pRSF-2 pRSFDuet-1 carrying tktA, aroB, aroE and aroK This study
 pRSF pRSFDuet-1 carrying tktA, aroGfbr, tyrAfbr, aroB, aroE and aroK This study
 pCDF-1 pCDFDuet-1 carrying RgTAL This study
 pCDF-2 pCDFDuet-1 carrying RgTAL, Ad4CL This study
 pCDF pCDFDuet-1 carrying RgTAL, Ad4CL, AdC2’H This study
 pCDF-Pc4CL1 pCDFDuet-1 carrying RgTAL, Pc4CL1, AdC2’H This study
 pCDF-Pt4CL pCDFDuet-1 carrying RgTAL, Pt4CL, AdC2’H This study
 pCDF-At4CL1 pCDFDuet-1 carrying RgTAL, At4CL1, AdC2’H This study
 pCDF-At4CL2 pCDFDuet-1 carrying RgTAL, At4CL2, AdC2’H This study