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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids

Fig. 1

Acceleration distribution testing of the vibration apparatus. a The schematic shows the plexiglass screen [1] secured to the 12″ subwoofer cone [2], and a superhydrophobic culture plate [3] attached to the screen via adhesive tape. An MP3 playback device [4] produces the sinusoidal signal at the desired frequency, with a power amplifier [5] to drive the sinusoidal signal through the subwoofer. b-d Schematics showing positions of amplitude measurements on (b) the plexiglass screen (number indicate distance in mm from centre), (c) the cell culture plate and (d) plates stacked on top of each other. Graphs illustrate (e) distribution testing on the plexiglass screen of the rig (*** = p < 0.001), (f) plate distribution testing (** = p < 0.01 and *** = p < 0.001), and (g) plate stacking amplitude testing (**** = p < 0.0001). Data are mean ± SEM (One-Way ANOVA with Tukey’s Multiple Comparison test). MEL: Middle Edge Long; MES: Middle Edge Short

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Journal of Biological Engineering

ISSN: 1754-1611