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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids

Fig. 2

Vibration increases the size of spheroids generated from NLMs. a-e: Representative images of spheroids vibrated at test frequencies. a 0 Hz control, (b) 20 Hz, (c) 60 Hz, (d) 80 Hz, (e) 80 Hz frequency “fractured” spheroid with cell debris (arrow). f-g Bar graphs show (f) spheroid number and (g) spheroid cross-sectional area per NLM (n = 10 NLMs per test condition, 3 repeats). *** = p < 0.001 in both F and G compared to control, and each test condition compared to each other. Data presented are mean ± SEM (Kruskal-Wallis test and Dunn’s post-hoc test for multiple comparisons). Scale bars = 500 μm in a-d, 250 in e

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Journal of Biological Engineering

ISSN: 1754-1611