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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction

Fig. 5

Filter assay and luciferase analysis of probes with alterations at the nonconsensus base pairs. a: List of the probes with alternations at nonconsensus base pairs. b: The binding intensities of the probes were measured with filter assay using lysates prepared from cells treated with (grey) and without (open) 10 μM arsenite for 1 h. c: Luciferase activities ratios of untreated to treated lysates with these probes were determined. Data shown are the mean values (±standard deviation) obtained from three independent experiments

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