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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Contribution of nonconsensus base pairs within ArsR binding sequences toward ArsR-DNA binding and arsenic-mediated transcriptional induction

Fig. 6

EMSA and luciferase analysis of reporter constructs with the binding sequences. a: Biotin-labeled probes of sECBS-CS9m, sECBS-CS12m and sECBS-CS15m were mixed with lysates prepared from untreated (open) and arsenic-treated cells (grey) and subjected to EMSA. b: Luciferase activities of sECBS-CS9m, sECBS-CS12m and sECBS-CS15m transformed cells pre-treated with or without 10 μM arsenite for 1 h were measured. c: Time course of luciferase induction of pECBS-CS12m (square) transformed cells pre-treated with 10 μM arsenite for 15, 30, 60 and 120 min were determined and compared with pECBS-AFBS (triangle) and pECBS-CS (diamond). Data shown are the mean values (±standard deviation) obtained from three independent experiments

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Journal of Biological Engineering

ISSN: 1754-1611