Fig. 4
From: Engineered mosaic protein polymers; a simple route to multifunctional biomaterials
Characterisation of Caf1 mosaic polymers by transmission electron microscopy (a) Schematic of the electron microscopy experiment. Mosaic Caf1 polymers containing a His tag (blue, Caf1His) and a biotinylated cysteine (yellow, Caf1Cys(Biotin)) are mixed with Nickel-NTA-10 nm and Streptavidin-20 nm gold conjugates. These recognise and bind to the His tags (blue triangles) and biotinylated cysteines (yellow semicircles) respectively. On the electron micrograph (grey box), these should appear as light grey beads (Caf1 polymer) surrounded by small and large black dots, representing the two different size gold conjugates. (b) Negative stain electron micrographs of the His-tagged, biotinylated cysteine containing mosaic polymer (Caf1His:Cys(Biotin)). His-tagged subunits were labelled with Nickel-NTA-10 nm gold particles and biotinylated cysteine containing subunits labelled with Streptavidin-20 nm gold particles. Images were taken at 92000x magnification. Dotted lines show the position of the Caf1 polymer. Additional data for WT unlabelled polymers are shown in Supplementary Information