Fig. 5
From: Engineered mosaic protein polymers; a simple route to multifunctional biomaterials
Caf1 mosaic polymers direct the early stages of bone formation. Phase microscopy images of primary human bone marrow stromal cells grown on plastic surfaces that were either uncoated (a) or coated with Caf1WT (b) or Caf1OPN:BMP2 (c) mosaic polymers (scale bar = 200 μm). Areas of mineralisation triggered by the differentiation of the cells are highlighted with black arrows. (d) qRT-PCR analysis of Runx2 (blue) and BMP2 (orange) expression from each of the cultures at 14 days. Error bars represent the standard deviation from three biological replicates