Fig. 7
From: Engineered mosaic protein polymers; a simple route to multifunctional biomaterials
Model of mosaic Caf1 polymer production by bacterial cells. A diagram of a bacterium is shown with the cytoplasm, periplasm and extracellular medium sections labelled. The cytoplasm contains the pCOP and pBad2x-Caf1OPN:BMP2 plasmids needed for Caf1 mosaic polymer production where genes are colour coded as follows: caf1R regulator (red), caf1M chaperone (green), caf1A usher (tan), caf1WT subunit (orange), caf1OPN mutant subunit (yellow) and caf1BMP2 mutant (cyan). Caf1M and Caf1 subunits are targeted to the periplasm and form chaperone:subunit complexes. These complexes are assembled into a Caf1 polymer by the Caf1A usher, which resides in the outer membrane. The usher does not discriminate between the different types of Caf1 subunit, and so the polymer formed contains a random mixture of the expressed subunits, and is thus a three-subunit mosaic heteropolymer