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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: A silkworm based silk gland bioreactor for high-efficiency production of recombinant human lactoferrin with antibacterial and anti-inflammatory activities

Fig. 2

Purification and secondary structure analysis of rhLF. a Purification of rhLF. M and WT represent the marker and wild type samples, respectively. Lane 1 represents the crude extract from cocoons. Lane 2 represents purified rhLF. Lane 3–5 represent 100, 250, 500 μg/ml of the rhLF-std, respectively. b Western blot analysis of the rhLF. c Detection rhLF after purification and dialysis. M represents the marker. Lanes 1–5 represent 5 batches of purified and dialyzed rhLF. d CD spectra of rhLF and rhLF-std. The rhLF and rhLF-std at the same concentration (0.5 mg/ml) were scanned within 190–250 nm

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