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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Development of a new promoter to avoid the silencing of genes in the production of recombinant antibodies in chinese hamster ovary cells

Fig. 2

Promoters containing RegCG are resistant to silencing in stable cell lines. Transcriptional activity in stable lines of CHO-K1 cells transfected with the reporter vectors and cloned by limiting dilution. The activity of the promoters was expressed in relative light units (RLU) calculated as the ratio between the luminescence and the concentration of total proteins in the cell lysates (Fir/Ren). In a) the decay of the activity of the cytomegalovirus (CMV) promoter in a group of clones is shown up to 41 days after the removal of the selection antibiotic (n = 12). In b) the activity of the promoters pGL-CMV, pGL-RegCG, pGL-RegCG/CMV, pGL-RegCG/GRE/CMV, pGL-RegCG/Enh/GRE/Core, and pGL-RegCG/GRE/Enh/GRE/Core of a group of randomly chosen clones on day 39 after selection was withdrawn (n = 16). Data are presented as mean values ± S.D. The one-tailed unpaired t-test, Mann-Whitney test, was used for statistical analysis. * p < 0.01 and ** p < 0.05

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