Fig. 5

Production of a recombinant antibody in CHO DG44 cells with a promoter containing RegCG. CHO DG44 cells were co-transfected with vectors expressing an anti -human tumor necrosis factor (TNF) antibody under the control of the chimeric promoter RegCG/GRE/CMV, subjected to genetic amplification by methotrexate; cell populations with higher production of antibodies were isolated by FACS. Clones were seeded in semi-solid medium, cultured in 96-well plates and their volumetric antibody production was analyzed by ELISA. a Schematic representation of pRegCG/GRE/CMV-aTNF-L and pRegCG/GRE/CMV-aTNF-H vectors to express the light and high chains of a human anti-TNF-L antibody. b The volumetric antibody production, viability, and specific productivity of the 7 highest production clones cultured in 125 mL flasks are shown. c The clone with the highest production level (clone number 7) was grown in a Spinner bottle. Cell density, viability, volumetric production, and specific productivity is shown. Representative result of three independent experiments