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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Intestinal probiotics E. coli Nissle 1917 as a targeted vehicle for delivery of p53 and Tum-5 to solid tumors for cancer therapy

Fig. 2

Efficient expression of p53 protein in E. coli BL21 (DE3) and probiotic EcN. a p53 expression levels in cell lysates (Pellet) or supernatants (Sup) were detected by subjecting E. coli BL21 (DE3) bearing p53-gene-expressing plasmid or empty plasmid (CK) to SDS-PAGE after IPTG induction and ultrasonication. b p53 protein could inhibit the proliferation of various types of cancer cells. PBS (CK) and 40 μg/mL recombinant p53 protein were coincubated with MCF-7, MDA-MB-231, Hep-3B, SMMC-7721, HCT-116, HeLa tumor cells, and CCK-8 solution was used to calculate the inhibitory effect of the recombinant protein on tumor cells. SDS-PAGE (c) and Western blot analysis (d) of p53 expression in EcN, EcN (28a), and EcN (p53)

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