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Fig. 3 | Journal of Biological Engineering

Fig. 3

From: Intestinal probiotics E. coli Nissle 1917 as a targeted vehicle for delivery of p53 and Tum-5 to solid tumors for cancer therapy

Fig. 3

Construction and expression of Tum 5-p53 bifunctional proteins. a Construction map of p53 and Tum-5 fusion expression vectors. T7: T7 promotor; SUMO: small ubiquitin-related

modifier; MMP: matrix metalloproteinase (MMP) cleavage site (PLGLWA); p18: the amino acid 50–67 of azurin. b Coomassie Brilliant Blue staining of SDS-PAGE showing fusion protein expression in E. coli BL21 (DE3). Recombinant proteins expression in cell lysates (Pellet) or supernatants (Sup) was detected by SDS-PAGE of E. coli BL21 (DE3) bearing recombinant plasmid after IPTG induction and ultrasonication

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