Fig. 4From: Intestinal probiotics E. coli Nissle 1917 as a targeted vehicle for delivery of p53 and Tum-5 to solid tumors for cancer therapyTum 5-p53 bifunctional protein was successfully expressed in tumor-targeting bacteria EcN. a Map of recombination plasmid containing Tum 5-p53 gene. Pvhb: promoter of the Vitreoscilla hemoglobin gene; pelB: the signal peptide from the pelB gene of Erwinia carotovora. SDS-PAGE (b) and Western blot analysis (c) of Tum 5-p53 bifunctional protein expression in EcN, EcN (28a), and EcN (Tum 5-p53)Back to article page