Fig. 5
From: Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates
Standards calibration curve of 5′ phosphorylated ssDNA. Column dimensions: XTerra® MS C18 2.5 μm, 4.6 mm × 50 mm. Ion pairing reagent: 0.1 M TEAA, pH 9.70 ± 0.05. Mobile phases: A; 5% acetonitrile in TEAA. B; 30% acetonitrile in TEAA. Gradient conditions: 90%A – 60%A in 16 min. 60–90% A in the next 14 min. Flowrate: 1.0 mL/min. Column and sample temperatures: 37 °C, and 33 °C. Injection volume: 100 μL. UV detector: 260 nm. Error bars represent ±1 SD of the peak areas from triplicate results