Skip to main content
Fig. 6 | Journal of Biological Engineering

Fig. 6

From: Characterization of covalent crosslinking strategies for synthesizing DNA-based bioconjugates

Fig. 6

Chromatographic results of the reactant ssDNA (a), and the phosphoramidated ssDNA conjugate (b). Column dimensions: XTerra® MS C18 2.5 μm, 4.6 mm × 50 mm. Ion pairing reagent: 0.1 M TEAA, pH 9.70 ± 0.05. Mobile phases: A; 5% acetonitrile in TEAA. B; 30% acetonitrile in TEAA. Gradient conditions: 90%A – 60%A in 16 min. 60–90% A in the next 14 min. Flowrate: 1.0 mL/min. Column and samples temperatures: 37 °C, and 33 °C. Injection volume: 100 μL. UV detector: 260 nm

Back to article page