Fig. 1

LCN2 is highly expressed in IS and may affect the proliferation and apoptosis of OGD-induced astrocytes. a the expression of LCN2 in the GSE30655 chip; *** p < 0.001. b, the expression of LCN2 in the GSE35338 chip; ** p < 0.01. c, the ability of cell repair determined by scratch test (scale bar = 100 μm); d, LIVE/DEAD staining (scale bar = 50 μm) and quantitative analysis for LDH release rate; e, cell proliferation detected by EdU staining (scale bar = 50 μm); f, TUNEL staining for astrocyte apoptosis (scale bar = 50 μm); g, Western blot analysis for protein bands and levels of inflammatory factors, proliferation and apoptosis marker proteins. The data were all expressed as mean ± standard deviation. The comparison between two groups was analyzed by non-paired t-test. The experiment was repeated three times. *, p < 0.05 vs. sh-LCN2-NC. IS, ischemic stroke; MCAO, middle cerebral artery occlusion model; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Bax, Bcl-2-associated X protein; Bcl-2, B-cell CLL/Lymphoma 2; IL-6, interleukin-6; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; sh-LCN2-NC, astrocytes transfected with sh-LCN2-NC plasmid; sh-LCN2, astrocytes transfected with sh-LCN2