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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Exosomes derived from microRNA-138-5p-overexpressing bone marrow-derived mesenchymal stem cells confer neuroprotection to astrocytes following ischemic stroke via inhibition of LCN2

Fig. 2

LCN2 is a target gene of miR-138-5p. a, predicted binding sites of miR-138-5p in 3’UTR of LCN2; b, luciferase activity of HEK293 cells detected by dual luciferase reporter gene assay; c, protein bands and quantitative analysis for LCN2 expression in astrocytes. The comparison among multiple groups was analyzed by one-way analysis of variance and the comparison between two groups was analyzed by non-paired t-test. The experiment was repeated three times. *, p < 0.05 vs. NC and mimic-NC. NC, negative control; WT, wide type; MUT, mutant type; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; mimic-NC, astrocytes transfected with mimic-NC; miR-138-5p mimic, astrocytes transfected with miR-138-5p mimic; inhibitor-NC, astrocytes transfected with inhibitor-NC; miR-138-5p inhibitor, astrocytes transfected with miR-138-5p inhibitor

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Journal of Biological Engineering

ISSN: 1754-1611