Fig. 4

Downregulation of miR-138-5p promotes apoptosis of astrocytes injured by OGD. a, the repair ability of damages in cells determined by scratch test (scale bar = 100 μm), and quantitative analysis for cell migration and the number of exosomes; b, LIVE / DEAD staining for LDH release rate (scale bar = 50 μm); c, cell proliferation detected by EdU staining and quantitative analysis for EDU positive expression (scale bar = 50 μm); d, TUNEL staining (scale bar = 50 μm) and quantitative analysis for TUNEL positive rate; e, expression of inflammatory factors, proliferation and apoptosis marker proteins determined by Western blot analysis. The data were all expressed as mean ± standard deviation. The comparison between two groups was analyzed by non-paired t-test. The experiment was repeated three times. *, p < 0.05 vs. inhibitor-NC. OGD, oxygen-glucose deprivation; LDH, lactate dehydrogenase; EdU, 5-Ethynyl-2′-deoxyuridine; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Bax, Bcl-2-associated X protein; Bcl-2, B-cell CLL/Lymphoma 2; IL-6, interleukin-6; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; inhibitor-NC, astrocytes transfected with inhibitor-NC plasmid; miR-138-5p-inhibitor, astrocytes transfected with miR-138-5p-inhibitor plasmid