Fig. 7

BMSCs deliver miR-138-5p to the astrocytes via the exosomes. a, quantitative analysis for the number of exosomes; b, the expression of the miR-138-5p in the co-culture system detected by RT-qPCR; c, repair ability of damages in cells determined by scratch test (scale bar = 100 μm), and quantitative analysis for cell migration and the number of exosomes; d, LIVE / DEAD staining for LDH release rate (scale bar = 50 μm); e, cell proliferation detected by EdU staining and quantitative analysis for EDU positive expression (scale bar = 50 μm); f, TUNEL staining (scale bar = 50 μm) and quantitative analysis for TUNEL positive rate; g, protein bands and quantitative analysis for expression of inflammatory factors, proliferation and apoptosis marker proteins determined by Western blot analysis. The data were all expressed as mean ± standard deviation. The comparison among multiple groups was analyzed by one-way analysis of variance. The experiment was repeated three times. *, p < 0.05 vs. DMSO or BMSCs-Control. LDH, lactate dehydrogenase; EdU, 5-Ethynyl-2′-deoxyuridine; TUNEL, terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; Bax, Bcl-2-associated X protein; Bcl-2, B-cell CLL/Lymphoma 2; IL-6, interleukin-6; IL-1β, interleukin-1β; TNF-α, tumor necrosis factor-α; BMSCs-control, astrocytes co-cultured with BMSCs-derived exosomes without any treatment; BMSCs-miR-NC, astrocytes co-cultured with BMSCs-derived exosomes infected with miR-NC; BMSCs-miR-138-5p, astrocytes co-cultured with BMSCs-derived exosomes infected with miR-138-5p overexpression; BMSCs, bone marrow-derived mesenchymal stem cells