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Fig. 7 | Journal of Biological Engineering

Fig. 7

From: Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Fig. 7

Crosslinking of cMTBSLS. Pre-reduced and desalted proteins (20 μM cMTBSLS or MTBSLS as a control) were incubated with 20 μM bis-maleimidoethane or buffer. Free thiol content in the proteins was assessed by quenching samples with 5-fluorosceinyl-maleimide (5F) prior to SDS-PAGE. Gels were imaged for fluorescence and then stained with Coomassie and imaged for total protein. The bands labeled with an asterisk represent crosslinked cMT (XLcMT)

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Journal of Biological Engineering

ISSN: 1754-1611