Table 1 Primers used in this study
Primer | Sequence | Restriction sites |
|---|---|---|
BEAS-S2131A-F | 5′-CCTGGAGATTGGAACCGCTACAGGTATGATCTTGTT-3′ | None |
BEAS-S2131A-R | 5′-ACATGCCCCGGTACATGACCGTCGCGTAGAGTAT-3′ | None |
BSLS-Bsu36I5518-F | 5′-AACCTCAGGATGCTGTCGATGCG-3′ | Bsu36I |
BSLS-without-MT-R | 5′-CACCGCCACACGTCGCTTTTCCGCAACCACAAATCCG-3′ | None |
BSLS-without-MT-F | 5′-CGGATTTGTGGTTGCGGAAAAGCGACGTGTGGCGGTG-3′ | None |
2nd-BSLS-B-R-PmlI | 5′-AACACGTGTAAAGACGCATTCAAAGCCT-3′ | PmlI |
MTBSLS-F-NheI | 5′-AAGCTAGCCACGACGACACTGCCGAACA-3′ | NheI |
MTBSLS-R-EcoRI | 5′-AAGAATTCTCACTGAAGTCGCTGGAGAGGTT-3′ | EcoRI |
MTBSLS-F-NdeI | 5′-AACATATGCACGACGACACTGCCGAACA-3′ | NdeI |
MTBSLS-R-PmeI | 5′-AAGTTTAAACTCACTGAAGTCGCTGGAGAGGTT-3′ | PmeI |
MTBEAS-F-NdeI | 5′-AACATATGGCTGACGATGCCGTTGAGCA-3′ | NdeI |
MTBEAS-R-PmeI | 5′-AAGTTTAAACCTGCAGCCGCTGCAGCGGCC-3′ | PmeI |
aMTBSLS-F-SpeI | 5′-AAACTAGTCAATTCAAGATTCGAAGTAACCGCATC-3′ | SpeI |
aMTBSLS-R-EcoRI | 5′-AAAGAATTCTCACGCCACAATGTGGGAAG-3′ | EcoRI |
cMTBSLS-F-NheI | 5′- AAAGCTAGCTGCGTTGCGGAACACG-3′ | NheI |
cMTBSLS-R-EcoRI | 5′-TTTGAATTCTCAGCACTGGAGAGGTTGATTGGTGAG-3′ | EcoRI |