Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Table 1 Primers used in this study

Primer	Sequence	Restriction sites
BEAS-S2131A-F	5′-CCTGGAGATTGGAACCGCTACAGGTATGATCTTGTT-3′	None
BEAS-S2131A-R	5′-ACATGCCCCGGTACATGACCGTCGCGTAGAGTAT-3′	None
BSLS-Bsu36I5518-F	5′-AACCTCAGGATGCTGTCGATGCG-3′	Bsu36I
BSLS-without-MT-R	5′-CACCGCCACACGTCGCTTTTCCGCAACCACAAATCCG-3′	None
BSLS-without-MT-F	5′-CGGATTTGTGGTTGCGGAAAAGCGACGTGTGGCGGTG-3′	None
2nd-BSLS-B-R-PmlI	5′-AACACGTGTAAAGACGCATTCAAAGCCT-3′	PmlI
MT_BSLS-F-NheI	5′-AAGCTAGCCACGACGACACTGCCGAACA-3′	NheI
MT_BSLS-R-EcoRI	5′-AAGAATTCTCACTGAAGTCGCTGGAGAGGTT-3′	EcoRI
MT_BSLS-F-NdeI	5′-AACATATGCACGACGACACTGCCGAACA-3′	NdeI
MT_BSLS-R-PmeI	5′-AAGTTTAAACTCACTGAAGTCGCTGGAGAGGTT-3′	PmeI
MT_BEAS-F-NdeI	5′-AACATATGGCTGACGATGCCGTTGAGCA-3′	NdeI
MT_BEAS-R-PmeI	5′-AAGTTTAAACCTGCAGCCGCTGCAGCGGCC-3′	PmeI
aMT_BSLS-F-SpeI	5′-AAACTAGTCAATTCAAGATTCGAAGTAACCGCATC-3′	SpeI
aMT_BSLS-R-EcoRI	5′-AAAGAATTCTCACGCCACAATGTGGGAAG-3′	EcoRI
cMT_BSLS-F-NheI	5′- AAAGCTAGCTGCGTTGCGGAACACG-3′	NheI
cMT_BSLS-R-EcoRI	5′-TTTGAATTCTCAGCACTGGAGAGGTTGATTGGTGAG-3′	EcoRI

ISSN: 1754-1611