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Table 2 Plasmids used in this study

From: Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Plasmid Description Restriction sites Source
pDY37 beas in YEpADH2p-URA3 NheI and PmlI [12]
pDY42 bsls in YEpADH2p-URA3 NdeI and PmlI [12]
pDY170 beas-G2131A in YEpADH2p-URA3 NheI and PmlI This work
pDY254 bsls(5818–9438)-Δmt in pJET1.2 Bsu36I and PmlI This work
pDY265 bsls-Δmt in YEpADH2p-URA3 Bsu36I and PmlI This work
pDY267 MTBEAS in pJET1.2 NdeI and PmeI This work
pDY268 MTBEAS in YEpADH2p-TRP1 NdeI and PmeI This work
pFC30 MTBSLS in pJET1.2 NdeI and PmeI This work
pFC31 MTBSLS in YEpADH2p-TRP1 NdeI and PmeI This work
pZJ135 MTBSLS in pJET1.2 NheI and EcoRI This work
pJCZ22 MTBSLS in pET28a NheI and EcoRI This work
paMTBSLS aMTBSLS in pET28a (a small portion of the A2 domain+MT of BSLS) NheI and EcoRI This work
pcMTBSLS cMTBSLS in pET28a (MT of BSLS containing an additional cysteine residue at both the N- and C-termini of the MT domain) NheI and EcoRI This work
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Journal of Biological Engineering

ISSN: 1754-1611