Modified substrate specificity of a methyltransferase domain by protein insertion into an adenylation domain of the bassianolide synthetase

Table 2 Plasmids used in this study

Plasmid	Description	Restriction sites	Source
pDY37	beas in YEpADH2p-URA3	NheI and PmlI	[12]
pDY42	bsls in YEpADH2p-URA3	NdeI and PmlI	[12]
pDY170	beas-G2131A in YEpADH2p-URA3	NheI and PmlI	This work
pDY254	bsls_(5818–9438)-Δmt in pJET1.2	Bsu36I and PmlI	This work
pDY265	bsls-Δmt in YEpADH2p-URA3	Bsu36I and PmlI	This work
pDY267	MT_BEAS in pJET1.2	NdeI and PmeI	This work
pDY268	MT_BEAS in YEpADH2p-TRP1	NdeI and PmeI	This work
pFC30	MT_BSLS in pJET1.2	NdeI and PmeI	This work
pFC31	MT_BSLS in YEpADH2p-TRP1	NdeI and PmeI	This work
pZJ135	MT_BSLS in pJET1.2	NheI and EcoRI	This work
pJCZ22	MT_BSLS in pET28a	NheI and EcoRI	This work
paMTBSLS	aMT_BSLS in pET28a (a small portion of the A₂ domain+MT of BSLS)	NheI and EcoRI	This work
pcMTBSLS	cMT_BSLS in pET28a (MT of BSLS containing an additional cysteine residue at both the N- and C-termini of the MT domain)	NheI and EcoRI	This work

ISSN: 1754-1611