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Fig. 2 | Journal of Biological Engineering

Fig. 2

From: Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Fig. 2

Identification of Pseudomonas sp. 102515. (a) A phylogenetic tree constructed based on the 16S rRNA sequences of Pseudomonas strains from BLASTn analysis. It estimates the relationship between strain 102515 and other Pseudomonas strains that shared the highest 16S rRNA gene sequence similarities. Numbers at the nodes indicate the normalized percentages of occurrence in 100 bootstrapped trees, and only values greater than 0.5 are shown. Sequences of reference species were obtained from GenBank, including P. luteola (NR_114215), P. asuensis (NR_136445), P. duriflava (NR_044390), P. benzenivorans (NR_116904), P.stutzeri (NR_041715), P. songnenensis (NR_148295), P. alcaligenes (NR_113646), P. otitidis (NR_043289), P. aeruginosa (NR_117678), P. alcaliphila (NR_114072), P. toyotomiensis (NR_112808), P. chengduensis (NR_125523), P.oleovorans (NR_043423), P. oryzihabitans (NR_114041), and P. psychrotolerans (NR_042191.1). (b) SEM micrograph of Pseudomonas sp. 102515. The bacterium was fixed with 2% glutaraldehyde in 0.1% HEPES buffer overnight. The samples were subjected to alcohol series dehydration (50–100% ethanol) and then chemically dried using hexamethyldisilazane

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