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Fig. 4 | Journal of Biological Engineering

Fig. 4

From: Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Fig. 4

Functional identification of PsCrtI as a phytoene desaturase. (a) HPLC analysis of lycopene production through co-expression of PsCrtI with the phytoene biosynthetic enzymes. (i) E. coli BL21(DE3)/pAC-PHYTipi (negative control) producing phytoene (retention time: 17.5 min, 280 nm), (ii) E. coli BL21(DE3)/pAC-PHYTipi+pOKF89 (pAC-PHYTipi+PsCrtI) producing lycopene (retention time: 14.8 min, 460 nm), (iii) E. coli BL21(DE3)/pAC-LYCipi (positive control) producing lycopene (retention time: 14.8 min, 460 nm). Color change of harvested cells due to the co-expression of PsCrtI with pAC-PHYTipi in E. coli BL21(DE3) is shown on the right. (b) A comparison of the UV spectra of lycopene produced by E. coli BL21(DE3)/pAC-PHYTipi+pOKF89 with lycopene produced by the positive control and phytoene produced by the negative control

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