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Table 2 Strains used in this study

From: Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Strain

Description

Source

E. coli XL1-Blue

endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F′[::Tn10 proAB+ lacIq Δ (lacZ)M15] hsdR17(rK− mK+)

Agilent Technologies

E. coli BL21(DE3)

BF− ompT gal dcm lon hsdSB (rB−mB−) λ (DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12(λS)

Novagen

Pseudomonas sp. 102515

Isolated endophytic strain from Taxus chinensis

This study

Pseudomonas putida KT2440

Heterologous host for expression of the Pscrt biosynthetic gene cluster

[76]

E. coli BL21(DE3)/pAC-PHYTipi

E. coli BL21(DE3) expressing the phytoene biosynthetic genes from pAC-PHYTipi (phytoene producing)

This study

E. coli BL21(DE3)/pAC-pHYTipi+pOKF89

E. coli BL21(DE3) expressing the phytoene biosynthetic genes from pAC-PHYTipi with PscrtI (lycopene producing)

This study

E. coli BL21(DE3)/pAC-LYCipi

E. coli BL21(DE3) expressing the lycopene biosynthetic genes from pAC-LYCipi (lycopene producing)

This study

E. coli BL21(DE3)/pAC-LYCipi+pOKF91

E. coli BL21(DE3) expressing the lycopene biosynthetic genes from pAC-LYCipi with PscrtY (β-carotene producing)

This study

E. coli BL21(DE3)/pAC-BETAipi

E. coli BL21(DE3) expressing the β-carotene biosynthetic genes from pAC-BETAipi (β-carotene producing)

This study

E. coli BL21(DE3)/pAC-ZEAXipi+pOKF72

E. coli BL21(DE3) expressing the β-carotene biosynthetic genes from pAC-ZEAXipi with CrtX (zeaxanthin diglucoside producing)

This study

E. coli BL21(DE3)/pAC-EHER

E. coli BL21(DE3) expressing the zeaxanthin diglucoside biosynthetic genes from pAC-EHER (zeaxanthin diglucoside producing)

This study

E. coli BL21(DE3)/pOKF184

E. coli BL21(DE3) expressing the Pscrt biosynthetic gene cluster from Pseudomonas sp. 102515

This study

Pseudomonas putida KT2440/pOKF192

Pseudomonas putida KT2440 expressing the Pscrt biosynthetic gene cluster from Pseudomonas sp. 102515

This study

Pseudomonas sp. 102515/ pOKF192

Engineered Pseudomonas sp. 102515 strain with an additional Pscrt biosynthetic gene cluster in pMIS1-mva

This study