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Table 2 Strains used in this study

From: Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Strain Description Source
E. coli XL1-Blue endA1 gyrA96(nalR) thi-1 recA1 relA1 lac glnV44 F′[::Tn10 proAB+ lacIq Δ (lacZ)M15] hsdR17(rK mK+) Agilent Technologies
E. coli BL21(DE3) BF ompT gal dcm lon hsdSB (rBmB) λ (DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB+]K-12S) Novagen
Pseudomonas sp. 102515 Isolated endophytic strain from Taxus chinensis This study
Pseudomonas putida KT2440 Heterologous host for expression of the Pscrt biosynthetic gene cluster [76]
E. coli BL21(DE3)/pAC-PHYTipi E. coli BL21(DE3) expressing the phytoene biosynthetic genes from pAC-PHYTipi (phytoene producing) This study
E. coli BL21(DE3)/pAC-pHYTipi+pOKF89 E. coli BL21(DE3) expressing the phytoene biosynthetic genes from pAC-PHYTipi with PscrtI (lycopene producing) This study
E. coli BL21(DE3)/pAC-LYCipi E. coli BL21(DE3) expressing the lycopene biosynthetic genes from pAC-LYCipi (lycopene producing) This study
E. coli BL21(DE3)/pAC-LYCipi+pOKF91 E. coli BL21(DE3) expressing the lycopene biosynthetic genes from pAC-LYCipi with PscrtY (β-carotene producing) This study
E. coli BL21(DE3)/pAC-BETAipi E. coli BL21(DE3) expressing the β-carotene biosynthetic genes from pAC-BETAipi (β-carotene producing) This study
E. coli BL21(DE3)/pAC-ZEAXipi+pOKF72 E. coli BL21(DE3) expressing the β-carotene biosynthetic genes from pAC-ZEAXipi with CrtX (zeaxanthin diglucoside producing) This study
E. coli BL21(DE3)/pAC-EHER E. coli BL21(DE3) expressing the zeaxanthin diglucoside biosynthetic genes from pAC-EHER (zeaxanthin diglucoside producing) This study
E. coli BL21(DE3)/pOKF184 E. coli BL21(DE3) expressing the Pscrt biosynthetic gene cluster from Pseudomonas sp. 102515 This study
Pseudomonas putida KT2440/pOKF192 Pseudomonas putida KT2440 expressing the Pscrt biosynthetic gene cluster from Pseudomonas sp. 102515 This study
Pseudomonas sp. 102515/ pOKF192 Engineered Pseudomonas sp. 102515 strain with an additional Pscrt biosynthetic gene cluster in pMIS1-mva This study