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Table 3 Plasmids used in this study

From: Discovery and engineering of an endophytic Pseudomonas strain from Taxus chinensis for efficient production of zeaxanthin diglucoside

Plasmid Description Source
pJET1.2 Cloning vector Fermentas
pET28a(+) E. coli expression plasmid, pBR322, copy number: ~ 40 Novagen
pACYC184 E. coli expression plasmid, p15A, copy number: 15 ATCC 37033
pAC-PHYTipi Phytoene producing plasmid Addgene plasmid #: 53283
pAC-LYCipi Lycopene producing plasmid Addgene plasmid #: 53279
pAC-BETAipi β-carotene producing plasmid Addgene plasmid #: 53277
pAC-ZEAXipi Zeaxanthin producing plasmid Addgene plasmid # 53287
pAC-EHER Zeaxanthin diglucoside producing plasmid Addgene plasmid #: 53262
pMIS1-mva-ges Expression plasmid for Pseudomonas putida KT2440 that contains the mevalonic acid (MVA) pathway genes [72]
pOKF55 crtX gene cloned from pAC-EHER in pJET1.2 This study
pOKF63 crtX gene in pET28a(+) This study
pOKF69 crtX gene with the J23119 constitutive promoter and B0034 ribosome binding site in pJET1.2 This study
pOKF72 crtX gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+) This study
pOKF85 PscrtI gene cloned from Pseudomonas sp. 102515 in pJET1.2 This study
pOKF88 PscrtY gene cloned from Pseudomonas sp. 102515 in pJET1.2 This study
pOKF89 PscrtI gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+) This study
pOKF91 PscrtY gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+) This study
pOKF163 The Pscrt biosynthetic gene cluster fragment A in pJET1.2 This study
pOKF166 The Pscrt biosynthetic gene cluster fragment B in pJET1.2 This study
pOKF169 The Pscrt biosynthetic gene cluster in pJET1.2 This study
pOKF173 The Pscrt biosynthetic gene cluster in pET28a(+) This study
pOKF184 The Pscrt biosynthetic gene cluster in pACYC184 This study
pOKF187 tnbA gene cloned from Pseudomonas sp. 102515 in pJET1.2 This study
pOKF192 The Pscrt biosynthetic gene cluster in pMIS1-mva This study