Table 3 Plasmids used in this study

pJET1.2

Cloning vector

Fermentas

pET28a(+)

E. coli expression plasmid, pBR322, copy number: ~ 40

Novagen

pACYC184

E. coli expression plasmid, p15A, copy number: 15

ATCC 37033

pAC-PHYTipi

Phytoene producing plasmid

Addgene plasmid #: 53283

pAC-LYCipi

Lycopene producing plasmid

Addgene plasmid #: 53279

pAC-BETAipi

β-carotene producing plasmid

Addgene plasmid #: 53277

pAC-ZEAXipi

Zeaxanthin producing plasmid

Addgene plasmid # 53287

pAC-EHER

Zeaxanthin diglucoside producing plasmid

Addgene plasmid #: 53262

pMIS1-mva-ges

Expression plasmid for Pseudomonas putida KT2440 that contains the mevalonic acid (MVA) pathway genes

[72]

pOKF55

crtX gene cloned from pAC-EHER in pJET1.2

This study

pOKF63

crtX gene in pET28a(+)

This study

pOKF69

crtX gene with the J23119 constitutive promoter and B0034 ribosome binding site in pJET1.2

This study

pOKF72

crtX gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+)

This study

pOKF85

PscrtI gene cloned from Pseudomonas sp. 102515 in pJET1.2

This study

pOKF88

PscrtY gene cloned from Pseudomonas sp. 102515 in pJET1.2

This study

pOKF89

PscrtI gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+)

This study

pOKF91

PscrtY gene with the J23119 constitutive promoter and B0034 ribosome binding site in pET28a(+)

This study

pOKF163

The Pscrt biosynthetic gene cluster fragment A in pJET1.2

This study

pOKF166

The Pscrt biosynthetic gene cluster fragment B in pJET1.2

This study

pOKF169

The Pscrt biosynthetic gene cluster in pJET1.2

This study

pOKF173

The Pscrt biosynthetic gene cluster in pET28a(+)

This study

pOKF184

The Pscrt biosynthetic gene cluster in pACYC184

This study

pOKF187

tnbA gene cloned from Pseudomonas sp. 102515 in pJET1.2

This study

pOKF192

The Pscrt biosynthetic gene cluster in pMIS1-mva

This study