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Fig. 8 | Journal of Biological Engineering

Fig. 8

From: The LINC complex, mechanotransduction, and mesenchymal stem cell function and fate

Fig. 8

YAP nuclear translocation in response to dynamic tensile loading in MSCs requires strain transfer to the nucleus via nesprin-1G. MSCs were seeded on aligned nanofibers and exposed to dynamic tensile loading (3% strain at 1 Hz). a Nesprin-1 giant knockdown (N1G) compared to cells treated with non-targeting miRNA (miRneg). b The nuclear deformation index quantified for the N1G group compared to the control at varying strains applied. c YAP- and DAPI-stained nuclei with or without 30 min of dynamic tensile loading (DL). Scale bar = 25 μm. d The ratio of nuclear to cytoplasmic YAP content was increased by DL (control miRneg), which effect was suppressed by nesprin-1 knockdown (N1G). #: p < 0.05, *: p < 0.05, ***: p < 0.001, ****: p < 0.0001. Reprinted with permission from [57]

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