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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions

Fig. 1

Schematic for sandwich detection of yeast surface-displayed Fn3 binding to Zika virions. Anti-Zika human IgG conjugated with Alexa488 enables detection of Fn3-Zika virion binding interaction. Alexa 405-conjugated anti-myc antibody (IgY from chicken) binds to myc tag on Fn3 C-terminus and allows quantification of Fn3 surface display

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