Skip to main content
Fig. 4 | Journal of Biological Engineering

Fig. 4

From: De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions

Fig. 4

Representative flow cytometric dot plots for analysis of yeast-displayed Clone71 site-directed saturation mutant libraries. Dot plot data was used to identify libraries containing candidate improved affinity Fn3 mutants. Pink ellipses in dot plots for position 80 and position 82 saturation mutant libraries encase yeast populations lying above the bulk diagonal. Position 85 mutant library (rightmost panel) provides example of saturation mutant library devoid of candidate higher affinity virion binders. Induced yeast were mixed with uninduced yeast at a ratio of 1:10 to prevent ligand (Zika virion) depletion effects. Dot plots contain data for 250,000 analyzed yeast cells

Back to article page