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Fig. 5 | Journal of Biological Engineering

Fig. 5

From: De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against Virions

Fig. 5

Flow cytometric histogram overlays for yeast-displayed Fn3s binding to Zika virions. X-axes denote binding of surface-displayed Fn3s to Zika virions as quantified via sandwich detection using Alexa488-conjugated anti-Zika IgG. C71: Clone 71. Ab Only denotes yeast samples incubated with culture supernatant from naïve Vero cells prior to labeling with Alexa488-conjugated anti-Zika IgG secondary reagent. Left panel: Yeast incubated with Zika virion stock diluted 1:5 in PBS/0.3% BSA. Right panel: Yeast incubated with Zika virion stock diluted 1:20 in PBS/0.3% BSA. Histograms contain data for 30,000 analyzed yeast cells per sample

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Journal of Biological Engineering

ISSN: 1754-1611