Fig. 8From: De Novo Isolation & Affinity Maturation of yeast-displayed Virion-binding human fibronectin domains by flow cytometric screening against VirionsFree-in-solution Fn3 ELISA assay. a Assay schematic. b, c Sandwich detection of virions bound by immobilized IgG. ‘Nil Virus’ denotes incubation with blank assay buffer, rather than virions, prior to incubation with Fn3. ‘Nil mAb’ denotes coating of ELISA plate assay wells with BSA rather than anti-Zika IgG. Error bars denote standard deviations for duplicate absorbance measurementsBack to article page