Fig. 2

Results of the expansion of TCLab and Gibco hiPSCs with mTeSR1 in the PBS MINI 0.1 bioreactor. At day 0, the reactor was inoculated with 1.5 × 10⁷ cells (2.5 × 10⁵ cells∙mL⁻¹) and 80% of the culture medium was changed daily starting from 48 h post-inoculation. a-d Representative images of cell aggregates harvested from the bioreactor, analysed through brightfield microscopy at days 1 and 7 of culture for a TCLab and c Gibco hiPSCs (scale bars = 250 μm) and through fluorescence microscopy at day 7 of culture culture for b TCLab and d Gibco hiPSCs following staining with calcein AM (scale bar = 100 μm). e Growth kinetics of the cells, in terms of total cell number and fold increase. f-i Aggregate size dynamics of the cells, in terms of f average aggregate diameter, g coefficient of variation, and aggregate size distribution profiles of a representative experiment at days 1, 4 and 7 post-inoculation for h TCLab and i Gibco hiPSCs. j-l Supernatant analysis of j glucose consumption and k lactate production, and l yield of lactate from glucose