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Table 1 Main results for all different tested conditions

From: Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors

Cell line

Culture medium

Fold change in cell number (day 1)

Maximum number of cells (cells/vessel)

Maximum cell density (cells∙mL− 1)

Maximum fold increase

Maximum specific growth rate (d− 1)

Minimum doubling time (d)

Volumetric productivity (cells∙mL− 1∙d− 1)

Maximum aggregate diameter (μm)

Minimum coefficient of variation

TCLab

mTeSR1

0.40 ± 0.03

(7.1 ± 0.7) × 107

(1.2 ± 0.1) × 106

4.8 ± 0.5

0.69 ± 0.13

1.1 ± 0.2

(1.7 ± 0.1) × 105

409 ± 25

28 ± 6%

Gibco

mTeSR1

0.27 ± 0.08

(6.2 ± 1.1) × 107

(1.0 ± 0.2) × 106

4.1 ± 0.7

0.66 ± 0.05

1.06 ± 0.09

(1.4 ± 0.3) × 105

338 ± 27

19.6 ± 0.6%

TCLab

mTeSR3D

0.24 ± 0.03

(6.1 ± 0.7) × 107

(8.8 ± 1.6) × 105

4.0 ± 0.5

1.1 ± 0.1

0.63 ± 0.06

(1.2 ± 0.1) × 105

367 ± 18

27 ± 2%

TCLab

mTeSR1 + DS

0.38 ± 0.06

(1.4 ± 0.1) × 108

(2.3 ± 0.2) × 106

9.3 ± 0.6

1.1 ± 0.2

0.64 ± 0.11

(4.6 ± 0.3) × 105

346 ± 11

20 ± 1%

TCLab

mTeSR3D + DS

0.50 ± 0.07

(1.26 ± 0.02) × 108

(1.79 ± 0.03) × 106

8.4 ± 0.1

0.96 ± 0.10

0.74 ± 0.09

(2.99 ± 0.05) × 105

347 ± 39

19 ± 2%