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Table 1 Main results for all different tested conditions

From: Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors

Cell line Culture medium Fold change in cell number (day 1) Maximum number of cells (cells/vessel) Maximum cell density (cells∙mL− 1) Maximum fold increase Maximum specific growth rate (d− 1) Minimum doubling time (d) Volumetric productivity (cells∙mL− 1∙d− 1) Maximum aggregate diameter (μm) Minimum coefficient of variation
TCLab mTeSR1 0.40 ± 0.03 (7.1 ± 0.7) × 107 (1.2 ± 0.1) × 106 4.8 ± 0.5 0.69 ± 0.13 1.1 ± 0.2 (1.7 ± 0.1) × 105 409 ± 25 28 ± 6%
Gibco mTeSR1 0.27 ± 0.08 (6.2 ± 1.1) × 107 (1.0 ± 0.2) × 106 4.1 ± 0.7 0.66 ± 0.05 1.06 ± 0.09 (1.4 ± 0.3) × 105 338 ± 27 19.6 ± 0.6%
TCLab mTeSR3D 0.24 ± 0.03 (6.1 ± 0.7) × 107 (8.8 ± 1.6) × 105 4.0 ± 0.5 1.1 ± 0.1 0.63 ± 0.06 (1.2 ± 0.1) × 105 367 ± 18 27 ± 2%
TCLab mTeSR1 + DS 0.38 ± 0.06 (1.4 ± 0.1) × 108 (2.3 ± 0.2) × 106 9.3 ± 0.6 1.1 ± 0.2 0.64 ± 0.11 (4.6 ± 0.3) × 105 346 ± 11 20 ± 1%
TCLab mTeSR3D + DS 0.50 ± 0.07 (1.26 ± 0.02) × 108 (1.79 ± 0.03) × 106 8.4 ± 0.1 0.96 ± 0.10 0.74 ± 0.09 (2.99 ± 0.05) × 105 347 ± 39 19 ± 2%