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Table 2 Comparison of aggregate-based suspension culture set-ups for the expansion of hPSCs

From: Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors

Cell type Culture medium Bioreactor type/volume Inoculum cell density (cells∙mL−1) Days in culture Maximum cell density (cells∙mL− 1) Volumetric productivity (cells∙mL− 1∙d− 1) Reference
hESCs mTeSR1 Spinner flask (100 mL) 1.8 × 104 6 4.5 × 105 7.5 × 104 [11]
hESCs mTeSR1 Spinner flask (50 mL) 1 × 106 7 2.4 × 106 3.4 × 105 [12]
hESCs StemPro hESC SFM Spinner flask (60 mL) 2.5 × 105 3–4 (1.1 ± 0.1) × 106 (3.7 ± 0.3) × 105 [13]
hiPSCs E8 Spinner flask (45 mL) (4-5)×105 3–4 (1.8 ± 0.3) × 106 (5.8 ± 0.8) × 105 [14]
hESCs mTeSR1 Spinner flask (100 mL) 2 × 104 6 (2.4 ± 0.09) × 105 (4.0 ± 0.2) × 104 [15]
hiPSCs mTeSR1/E8 Stirred tank (125 mL) 5 × 105 7 (2.85 ± 0.34) × 106 (4.07 ± 0.49) × 105 [10]
hiPSCs mTeSR1 Vertical-Wheel (60 mL) 2.5 × 105 7 (1.2 ± 0.1) × 106 (1.7 ± 0.1) × 105 This study
hiPSCs mTeSR1 + DS Vertical-Wheel (60 mL) 2.5 × 105 7 (2.3 ± 0.2) × 106 (4.6 ± 0.3) × 105 This study