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Table 2 Comparison of aggregate-based suspension culture set-ups for the expansion of hPSCs

From: Strategies for the expansion of human induced pluripotent stem cells as aggregates in single-use Vertical-Wheel™ bioreactors

Cell type

Culture medium

Bioreactor type/volume

Inoculum cell density (cells∙mL−1)

Days in culture

Maximum cell density (cells∙mL− 1)

Volumetric productivity (cells∙mL− 1∙d− 1)

Reference

hESCs

mTeSR1

Spinner flask (100 mL)

1.8 × 104

6

4.5 × 105

7.5 × 104

[11]

hESCs

mTeSR1

Spinner flask (50 mL)

1 × 106

7

2.4 × 106

3.4 × 105

[12]

hESCs

StemPro hESC SFM

Spinner flask (60 mL)

2.5 × 105

3–4

(1.1 ± 0.1) × 106

(3.7 ± 0.3) × 105

[13]

hiPSCs

E8

Spinner flask (45 mL)

(4-5)×105

3–4

(1.8 ± 0.3) × 106

(5.8 ± 0.8) × 105

[14]

hESCs

mTeSR1

Spinner flask (100 mL)

2 × 104

6

(2.4 ± 0.09) × 105

(4.0 ± 0.2) × 104

[15]

hiPSCs

mTeSR1/E8

Stirred tank (125 mL)

5 × 105

7

(2.85 ± 0.34) × 106

(4.07 ± 0.49) × 105

[10]

hiPSCs

mTeSR1

Vertical-Wheel (60 mL)

2.5 × 105

7

(1.2 ± 0.1) × 106

(1.7 ± 0.1) × 105

This study

hiPSCs

mTeSR1 + DS

Vertical-Wheel (60 mL)

2.5 × 105

7

(2.3 ± 0.2) × 106

(4.6 ± 0.3) × 105

This study