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Table 2 Comparison of High Five™ cells cultured in 300-mL shake flasks with different commercially available culture media

From: Process development for pandemic influenza VLP vaccine production using a baculovirus expression system

Name of commercial medium Seeding cell density (cells/mL) Harvested cell density (cells/mL) Cell doubling time (hour) Fold increase of cell density HA titer (HAU/50 μL)
HyClone™ SFM4Insect 2.00 × 105 2.60 × 106 19.46 13.00 512
EX-CELL® 405 2.00 × 105 1.80 × 106 22.71 9.00 256
Esf-921 2.05 × 105 2.25 × 106 20.83 10.98 256
Express Five® SFM 2.16 × 105 3.30 × 106 18.30 15.28 256