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Table 2 Comparison of High Fiveâ„¢ cells cultured in 300-mL shake flasks with different commercially available culture media

From: Process development for pandemic influenza VLP vaccine production using a baculovirus expression system

Name of commercial medium

Seeding cell density (cells/mL)

Harvested cell density (cells/mL)

Cell doubling time (hour)

Fold increase of cell density

HA titer (HAU/50 μL)

HyCloneâ„¢ SFM4Insect

2.00 × 105

2.60 × 106

19.46

13.00

512

EX-CELL® 405

2.00 × 105

1.80 × 106

22.71

9.00

256

Esf-921

2.05 × 105

2.25 × 106

20.83

10.98

256

Express Five® SFM

2.16 × 105

3.30 × 106

18.30

15.28

256