Process development for pandemic influenza VLP vaccine production using a baculovirus expression system

Table 3 Comparison of H7N9-TW VLP production in a 5-L bioreactor with different control parameters

Parameter/Group	1	2	3
Cell density (10⁶ cells/mL)^a	2.0	8.0	2.4
DO (%)	30	30	80
pH	6.4	6.4	6.4
Agitation rate (rpm)^b	120–180	120–180	120–180
HA titer (HAU/50 μL)	8	< 2	512

High Five™ cells were cultured in a 5-L bioreactor with different control parameters, infected with the baculovirus and harvested at 3 days postinfection
^aThe cell density value indicates the cell density at which baculovirus infection was performed
^bThe agitation rate was adjusted depending on the cell density during culture

ISSN: 1754-1611