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Table 3 Comparison of H7N9-TW VLP production in a 5-L bioreactor with different control parameters

From: Process development for pandemic influenza VLP vaccine production using a baculovirus expression system

Parameter/Group 1 2 3
Cell density (106 cells/mL)a 2.0 8.0 2.4
DO (%) 30 30 80
pH 6.4 6.4 6.4
Agitation rate (rpm)b 120–180 120–180 120–180
HA titer (HAU/50 μL) 8 <  2 512
  1. High Five™ cells were cultured in a 5-L bioreactor with different control parameters, infected with the baculovirus and harvested at 3 days postinfection
  2. aThe cell density value indicates the cell density at which baculovirus infection was performed
  3. bThe agitation rate was adjusted depending on the cell density during culture