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Fig. 1 | Journal of Biological Engineering

Fig. 1

From: Recombinant baculovirus expressing the FrC-OVA protein induces protective antitumor immunity in an EG7-OVA mouse model

Fig. 1

Construction of the BV transfer vector and FrC-OVA expression DNA vaccine. a Construction of the FrC-OVA-pAc-CAG-MCS2 and FrC-OVA-pVAX1-CAG-MCS plasmids containing the gene encoding the first domain of the FrC of tetanus toxin (TT865–1120). These plasmids were constructed to encode OVA 257–269 peptides fused directly to FrC. b Expression of the OVA protein in HEK-293 T cells infected with rBV or transfected with FrC-OVA-pVAX1-CAG-MCS. The cell extracts were separated by SDS-PAGE and analyzed by immunoblot using an anti-OVA antibody. Lane 1, transfected with OVA-pcDNA3.1 using FuGEN6; lane 2, transfected with FrC-OVA-pVAX1-CAG-MCS using FuGENE-6, lane 3, infected with rBV at an MOI of 100

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