Fig. 9
From: A novel egg-shell membrane based hybrid nanofibrous scaffold for cutaneous tissue engineering
(a) Survival analysis of cells (MTT assay) on days 7 and 14. Data showed a significant increase in viability after plating on PCL/SF/SESM and PCL/SF/SESM/AV compared to matched-control groups at day 7 (***p < 0.001). This study was performed sextuplicate (n = 6). (b) SEM imaging of BCCs on the different nanofibers on days 7 and 14. It is shown that BCCs could easily attach the nanofiber surface and determined by a flattened appearance. In scaffolds such as PCL/SF/SESM and PCL/SF/SESM/AV cells tended to tightly connect with each other 14 days after plating. These features showed the ability of cultured cells to generate a uniform cell monolayer. This study was performed triplicate (n = 3). Arrow heads: cell margin; arrows cell mass