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Fig. 11 | Journal of Biological Engineering

Fig. 11

From: An overview of advanced biocompatible and biomimetic materials for creation of replacement structures in the musculoskeletal systems: focusing on cartilage tissue engineering

Fig. 11

Fluorescent immunocytochemistry tests and quantification of Pax7, MyoD, and myogenin. (a) Muscle stem cells in Matrigel and collagen-I coatings were stained for Pax7, MyoD, or myogenin (all green) together with actin (red) and DAPI (blue) after differentiation. (b) Quantification of Pax7+, MyoD+, and myogenin+ cells (expressed as a mean ± SD) in Matrigel and collagen-I coatings after differentiation. Scale bar represents 50 μm. Significant difference between the Matrigel and collagen-I. Republished with permission of ref. [112], Grefte S, Vullinghs S, Kuijpers-Jagtman A, Torensma R, Von den Hoff J. Matrigel, but not collagen I, maintains the differentiation capacity of muscle-derived cells in vitro. Biomedical materials. 2012;7(5):055004, Copyright (2019)

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