Fig. 4

Immunofluorescence assay of 3D co-cultures using the 3D-tip. Co-culture of astrocytes detected by anti-GFAP (green) and S100β-DsRed Schwann cells (red). Cells were stained with Hoechst (blue). Scale bar = 100 μm. Images were captured on an Olympus FV1000 confocal microscope