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Fig. 6 | Journal of Biological Engineering

Fig. 6

From: 3D-tips: user-friendly mesh barrier pipette tips for 3D-spheroid culture

Fig. 6

Comparison between the 3D-tip and the centrifugation methods for fluorescence labelling of live (unfixed) 3D OEC cultures. a Representative images of unfixed 3D OEC spheroids stained with Hoechst (blue) and CellMask Deep Red (red) using the 3D-tip and the centrifugation methods. b Sphericity analysis of 3D structures after the staining assay using the 3D-tip and the centrifugation methods. c Percentage of spheroids remaining after the staining assay using the 3D-tip and the centrifugation methods. Scale bar = 50 μm. Images were captured on an Olympus IX73 microscope. The analysis was performed using AnaSP 1.0. p*< 0.05, p**< 0.01, n = 3, technical replicates

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